Soliman Khatib
Tel-Hai College, Israel
Title: Lyso-DGTS lipid isolated from microalgae enhances PON1 activities in vitro and in vivo, increases PON1 penetration into macrophages and decreases cellular lipid accumulation
Biography
Biography: Soliman Khatib
Abstract
High-density lipoprotein (HDL) plays an important role in preventing atherosclerosis. The antioxidant effect of HDL is mostly associated with paraoxonase 1 (PON1) activity. Increasing PON1 activity using natural comounds may improve HDL functions and decrease atherosclerotic risk. In a previous study we isolated the compound, lyso-DGTS (C20:5,0) from Nannochloropsis sp. ethanol extract. In the present study, the effect of lyso-DGTS on PON1 activities was examined and the mechanism by which the compound affects PON1 activity was explored. Lyso-DGTS increased and preserved recombinant PON1 (rePON1) and human serum PON1 activities in a dose dependent manner. Tryptophan-fluorescence-quenching assay and molecular modeling calculations, showed a spontaneous lyso-DGTS - rePON1 interaction which supported by hydrogen bonds and van der Waals interactions. Furthermore, Lyso-DGTS increased peneteration of rePON1 into macrophages and prevented macrophages from lipid accumulation after stimulation with oxidized low-density lipid (ox-LDL). In-vivo experiment show that Lyso-DGTS significantly increased PON1 lactonase activity and decreased glucose concentrations in a serum of mice fed a high-fat diet to the level of mice fed a normal diet. Our findings suggest a beneficial effect of lyso-DGTS on increasing PON1 activity and thus, improving HDL quality and atherosclerotic risk factors.